Figure 9. Flow cytometric analysis for CD3⁺CD4⁺ splenic T lymphocyte population.

(A) Representative flow cytometry scatter dot plots for CD3⁺, CD3⁺CD4⁺, CD3⁺CD8⁺ splenic T cell populations of immunized mice (n = 5) and the negative controls (NC) (n = 5). The subpopulations are expressed as a percentage of the gated cells. II) CD3⁺, CD3⁺CD4⁺ and CD3⁺CD8⁺ splenic T-cell subsets in immunized mice and NC. The data are presented as the mean ± s.d. *P < 0.05 when the values are compared with the negative control group.