| E. coli |
| DH5α |
fhuA2 Δ(argF-lacZ)U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17 |
Lab stock |
| BL21(DE3) |
139(ara-leu)7697 galUgalKrpsL (Strr)endA1 nupGF−ompThsdSB(rB−mB−)dcmgalλ(DE3) pLysSCmr |
Lab stock |
| χ6212 |
F-λ-φ80 Δ(lacZYA-argF) endA1 recA1 hadR17 deoR thi-1 glnV44 gyrA96 relA1 ΔasdA4
|
Lab stock |
|
Salmonella Typhimurium |
| JOL401 |
Wild typeisolate from chicken |
Lab stock |
| JOL990 |
Wild typeisolate from porcine, challenge strain |
Lab stock |
| JOL1311 |
Δasd, used as base vaccine strain |
Lab stock |
| JOL1754 |
JOL1311 containing pJHL172 |
This study |
| JOL1954 |
JOL1311 containing pJHL420 |
This study |
| Plasmids |
| pJHL319 |
T-easy vector harboring E gene ghost cassette with the convergent promoter system |
This study |
| pJHL350 |
T-easy vector harboring E gene and the R ghost cassette with the convergent promoter system |
This study |
| pJHL172 |
asd+vector, pBRoriplasmid harboring cI857/λPR promoter, araC ParaBAD, phiX174 lysis gene E
|
8 |
| pJHL420 |
asd+vector, pBRoriplasmid harboring cI857/λPR promoter, araC ParaBAD, phiX174 lysis gene E, the R ghost cassette composed of S, R and R1/Rzgenes |
This study |
