Figure 2. Chronic nicotine modifies alcohol intake.

(a) Mean ± SEM of ethanol consumption (gr/kg) in nic− (black, n = 18) and nic+ (gray, n = 15) mice during the two bottle choice procedure. Horizontal lines indicate significant dose effect and vertical lines indicate treatment effect. ***p < 0.001, **p < 0.01, repeated measures two-way ANOVA. (b) Mean ± SEM of the preference ratio for alcohol over total fluid intake for the same groups of mice. ***p < 0.001, **p < 0.01, repeated measures two-way ANOVA; (c) Mean ± SEM of the total fluids intake for the same groups of mice. (d) Barplot of the total alcohol intake (within the 18 days procedure) for the same groups. **p < 0.01, Wilcoxon test.