Table 1. Model parameters for recombinant pET-based E. coli BL21(DE3) strains producing GFP, HRP, the scFv or the tadem scFv.
rec. product | qs,lac,max* [g/g/h] | KA [g/g/h] | qs,glu,crit [g/g/h] | n [-] | qs,lac,noglu [g/g/h] | NRMSE [%] |
---|---|---|---|---|---|---|
GFP | 0.23 | 0.0042 | 0.88 | 1.77 | 0.023 | 0.04 |
HRP | 0.17 | 0.0092 | 1.06 | 1.15 | 0.0032 | 5.14 |
scFv | 0.09 | 0.019 | 0.88 | 1.16 | 0.034 | 9.72 |
tandem scFv | 0.13 | 0.094 | 1.02 | 1.48 | 0.040 | 9.11 |
The mechanistic model is shown in Equation (1).