Table 1. Primers and probes summary.

Gene	Abbreviation	Forward (5′–3′)	Riverse (5′–3′)	Probe (5′FAM/3′TAMRA)
Collagen type I	COL 1	CCC TGG AAA GAA TGG AGA TGA T	ACT GAA ACC TCT GTG TCC CTT CA	CGG GCA ATC CTC GAG CAC CCT
Collagen type II	COL 2	GGC AAT AGC AGG TTC ACG TAC A	GAT AAC AGT CTT GCC CCA CTT ACC	CCT GAA GGA TGG CTG CAC GAA ACA TAC
Collagen type X	COL 10	ACG CTG AAC GAT ACC AAA TG	TGC TAT ACC TTT ACT CTT TAT GGT GTA	ACT ACC CAA CAC CAA GAC ACA GTT CTT CAT TCC
Aggrecan	ACAN	AGT CCT CAA GCC TCC TGT ACT CA	CGG GAA GTG GCG GTA ACA	CCG GAA TGG AAA CGT GAA TCA GAA TCA ACT
Gene	Abbreviation	Applied Biosystems Serial number
SYR box-9	SOX 9	Hs_00165814_m1
ribosomal 18 S	18 S	4310893E