Figure 3. Reduced allergic symptoms in mice exposed to acrolein are associated with an increase in the Foxp3/CD3 ratio in the lung.

(A) Mice nasally treated with KLH (K), acrolein (A), PBS (P) or with KLH in combination with acrolein (KA) were exposed to increasing doses of methacholine and the degree of airway hyperreactivity was measured by whole body plethysmography according to change in area of enhanced pause (Penh). (B) Rectal temperature drop 20 min after ip-challenge with KLH as a parameter to determine systemic anaphylactic reactions (C) Summary of CD4+CD25+Foxp3+ cell numbers counted in the spleens of the different treated mice of two independently performed experiments. (D) Whole lung sections were imaged by TissueFAX and positive stainings were quantified by Histoquest. Summary of immune-histochemical quantification % of CD3+ cells, (D) % of Foxp3+ cells (E) Foxp3/CD3 ratio of lung sections in the different treated groups. (F) Representative regions of lung sections stained for CD3 and Foxp3. 2 stained sections per animal were analysed by TissueFAX. Groups were compared by ANOVA following Newman-Keuls Multiple Comparison test. Mean ± SEM; *P < 0.05; **P < 0.01; ***P < 0.001.