Figure 3.

ELISA binding of scFv monoclonals with HIV-1 envelope glycoproteins: ELISA was done to determine binding specificity of the purified scFv monoclonals (at 10 μg/ml concentration) with (a) h-CMP-V1cyc1 gp120 (b) with consensus C gp120 (c) and with trimeric BG505-SOSIP.664 gp140. HEP scFv, the scFv against the hepatitis antigen⁴⁸ served as negative control for all the assays. (d) ELISA binding reactivity of scFv monoclonals (at 10 μg/ml concentration) with V3C and V3B peptides was tested. B6, an anti-V3 scFv generated in our lab earlier (unpublished data), is used as positive control. Mean binding titres were compared with negative control using un-paired t test. P values < 0.05 were considered as significant.