Table 3. N332A glycan directed neutralization activity of C11 scFv.
| Viruses | Neutralization Titers IC50(ug/ml) | Fold IC50 (ug/ml) increase relative to wild type |
||||
|---|---|---|---|---|---|---|
| C11 | 1F6 | D11 | C11 | 1F6 | D11 | |
| CAP256-WT | 20.8 | >50 | 42.73 | — | — | — |
| CAP256-N332A | >50 | >50 | 35.38 | >2.4 | — | 0.8279 |
N332A dependent neutralization activity (IC50 values) of scFv monoclonals. The scFv monoclonals were checked for their neutralizing efficiency with virus CAP256 wild type and its N332A mutant50,51, at concentrations ranging from 50 μg/ml to 3.125 μg/ml. HEP scFv48 was taken as negative control. MuLV was used as a negative control along with virus controls and cell controls. Non-linear regression curve straight line was plotted using the method of least squares to determine the IC50 values.