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. 2017 Mar 23;7:45155. doi: 10.1038/srep45155

Figure 4. Confocal imaging of TRPM8, NFκB and TNFα expression in PC12 cells.

Figure 4

(A) The expression of TRPM8, NFκB and TNFα in wild type cells and Trpm8 knockdown cells at 37 °C and 4 °C. KD signifies Trpm8 knockdown cells. (B) In wild type cells, TRPM8 was upregulated and NFκB and TNFα were downregulated under cold conditions. (C) In KD cells, TRPM8 showed weak expression and NFκB and TNFα expression levels were increased. (D) Co-localization of TRPM8 and TNFα in the cytoplasm (cold condition and 500 nM menthol). Data are shown as the mean ± S.D. from three experiments. *P < 0.05; **P < 0.01.