Figure 5. CoCl2-induced hypoxia-like stress upregulates DDIT4 expression independent of PML.

(A) Quantitative PCR analysis of DDIT4 mRNA expression in shControl and shPML cells treated with 500 μm CoCl₂ for 24 h (n = 4). (B) Western blot analysis of DDIT4 expression in shControl and shPML U2OS cells treated with 500 μm CoCl₂ for 24 h. (C) Relative DDIT4 expression in CoCl₂-treated U2OS from cells treated as in A (n = 6). (D) Western blot analysis and densitometry of S6 and phospho(Ser235/236) S6 levels in shControl and shPML U2OS cells treated with 500 μm CoCl₂ for 24 h. Values in A and C represent the mean+/−SEM. p-values < 0.01 (**) or <0.05 (*) and n.s. = not significant. Full-length blots for this figure are presented in Supplementary Figure 5.