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. 2017 Mar 23;7:44988. doi: 10.1038/srep44988

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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MDCK-II cells with inducible expression of LOXL2-Flag were treated with doxycycline and after that with the IRE1 inhibitor 4 μ8 C for the indicated time periods. Cells were processed for: (a) WB using antibodies against LOXL2 (anti-Flag), E-cadherin (E-Cad), N-cadherin (N-cad), Fibronectin (FN) and Vimentin. α-tubulin was used as loading control. One representative blot of two independent experiments is shown. (b) Phase contrast image of the cells after 2 weeks of inhibitor treatment (right) compared to control untreated cells (left). (c) Representative images of confocal immunofluorescence analyses of control and 4 μ8 C treated cells for the indicated time periods with antibodies against LOXL2 (anti-Flag), E-cadherin (E-cad), ZO-1 and vimentin. F-actin was detected with phalloidin stain. Merge images are shown on the right panels. Scale bars 50 μm.