Figure 3.

Mito-CP induces apoptotic cell death. SK-MEL28 (A) and A375 (B) cells were treated with increasing doses of Mito-CP for 24 h prior to annexin V/propidium iodide staining. The graphs (right) indicate annexin V positive and propidium iodide positive cell populations. 50 µM cisplatin treatment for 24 h was used as a positive control to induce apoptosis. Cisplatin was dissolved in water. Data (mean ± SEM) are from a representative experiment performed in triplicate. *P < 0.05; ***P < 0.001.