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. 2017 Mar 23;15:60. doi: 10.1186/s12916-017-0817-5

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Immunohistochemical staining of TEX101 protein in testicular tissue with active spermatogenesis. a Testicular tissue stained with our monoclonal antibody 23ED228 (final concentration 80 ng/mL). Cell types presented include Sertoli cells (negative staining), Leydig cells (negative staining), and germ cells at different stages of spermatogenesis, such as spermatogonia (negative), primary spermatocytes (positive cytoplasm), secondary spermatocytes (positive membrane), spermatids (positive), and spermatozoa. b Negative control (no primary antibody added). c Hematoxylin and eosin staining of testicular tissue showing nucleus (purple) and cytoplasm (pink)