Table 1. Pre-steady-state kinetic constants for the incorporation of dTTP on a heteropolymeric template-primer by WT and mutant HIV-2 RTs.
| Enzyme | kpol (s−1) | Kd (μM) | kpol/Kd (μM−1·s−1) |
|---|---|---|---|
| WT HIV-2 RT | 13.6 ± 1.6 | 42.2 ± 11.1 | 0.32 ± 0.09 |
| K65R HIV-2 RT | 16.0 ± 1.1 | 53.7 ± 9.1 | 0.30 ± 0.06 |
| Q151M HIV-2 RT | 50.8 ± 4.7 | 99.0 ± 21.2 | 0.51 ± 0.12 |
| M184V HIV-2 RT | 27.5 ± 5.9 | 164 ± 77 | 0.168 ± 0.087 |
| K65R/Q151M/M184V HIV-2 RT | 19.1 ± 2.4 | 210 ± 53 | 0.091 ± 0.025 |
| WT BH10 HIV-1 RT | 11.6 ± 0.5 | 13.4 ± 1.9 | 0.86 ± 0.13 |
The template-primer 31T/21P was used as the substrate. Mean values ± standard deviations are given for each enzyme. Assays were performed independently at least three times. Reported values for the WT BH10 HIV-1 RT were taken from Matamoros et al.44.