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. Author manuscript; available in PMC: 2017 Mar 23.
Published in final edited form as: Anticancer Res. 2016 Mar;36(3):877–886.

Figure 4.

Figure 4

Antiproliferative effects of Gemini 20S-hydroxyvitamin D3 (20D3) analogs on human SKEML-188 melanoma cells and anti-inflammatory effects on splenocytes. A: SKEML-188 melanoma cells were treated with analogs, 1α,25-dihydroxyvitamin D3 (1,25D3) or dimethyl sulfoxide (DMSO) (solvent) as a control to assess their inhibitory effects on cell growth. Gemini 20D3 analogs reduced interferon-γ (IFNγ) concentration (B) and up-regulated leukocyte-associated immunoglobulin-like receptor 1 (LAIR1) levels (C) in mouse splenocytes. Splenic cells were treated by 100 nM of Gemini analogs, 1,25D3, 22-Oxa or EtOH only (solvent) as a control. Data are presented as mean±SE (n=3). *p<0.05, **p<0.01, ***p<0.005, ****p<0.001 compared to the control. Half-maximal inhibitory concentration (IC50): 1.65×10−8 M (10d), 1.24×10−9 M (10e) and 1.05×10−9 M (1,25D3).