Figure 5.

Treatment with 4-1BB Aptamer-CD25 siRNA Conjugates Enhances the Immunogenicity of Irradiated Tumors

(A–I) 4T1 tumor-bearing BALB/c mice were irradiated with 10 Gy. 5 days later, they were treated with 4-1BB-CD25 or 4-1BB-luc conjugates twice 2 days apart (4–5 mice/group). 10 days after irradiation, tumors were excised and analyzed by flow cytometry (see Materials and Methods). CD4⁺ Tconv cells, Foxp3 negative CD4⁺ T cells; Treg, Foxp3-expressing CD4⁺ T cells (4–5 mice/group; n = 2).