Figure 3.

Co-localization of Electrotransfected Plasmid DNA with Dextran

(A) The panel shows a representative confocal image of B16.F10 cells expressing Lifeact-GFP. The cells were treated with electric pulses in the presence of both tetramethylrhodamine-labeled dextran and Cy5-labeled pEGFP. Live images were taken at 10 min after electrotransfection. Pseudo colors were assigned to each channel for visualization: red for tetramethylrhodamine; green for EGFP; and blue for Cy5, which are shown in images C1, C2, and C3, respectively. The colors are merged in the fourth image. (B) The panel shows the characterization of co-localization by Manders’ co-localization coefficient (MCC). M1 represents the fraction of all pixels in a z stack of confocal images from channel 1 (red) that co-localized with those from channel 2 (blue). M2 represents the fraction of all pixels from channel 2 (blue) that co-localize with those from channel 1 (red); n = 9. The bar and error bar represent mean and SEM, respectively.