Figure 1.

Expression of miR-483-5p Is Elevated in the Articular Cartilage of OA Patients and Experimental OA Mice

(A) Real-time PCR analysis of miR-483-5p levels in human OA articular cartilage compared to controls. (B) Safranin O fast green staining of knee joints from DMM and sham mice. Black arrows indicate the tidemark. Scale bars represent 100 μm (top) and 40 μm (bottom). (C) OARSI scores according to safranin O and fast green staining in (B) (n = 5). (D) Real-time PCR analysis showed a significant increase in the levels of miR-483-5p in mouse OA articular cartilage (n = 5) compared to the sham surgery group (n = 5). Error bars represent the mean ± SEM. *p < 0.05; **p < 0.01 (independent-sample t test for two groups and one-sample t test for real-time PCR analysis).