Figure 5.

Vector Copy Numbers Correlate with Hemoglobin Level and the Counts of RBCs and WBCs in Thalassemic Mice Transplanted with γ-Globin/NA10HD Expressing Lentivirus

(A) Schematic diagram of the γ-globin/NA10HD lentiviral vector indicating the restriction enzyme sites used for Southern blot analysis. (B) Analysis of genomic DNA pattern of mice in γ-globin/NA10HD group as measured by Southern blot analysis. The DNA was digested using BglII, and the size of the DNA band is indicated by arrow. The copy number of each lane represents the vector copy number quantified by densitometry relative to the “standard one-copy”; a K562 clone containing a single copy of an integrated GFP lentiviral vector was used as the “standard one-copy” reference. (C–E) Correlations of copy number of individual animals of the γ-globin/NA10HD lentiviral vector with Hb level (C), RBC count (D), and WBC count (E).