Table 1.
Viral Vector γ-Globin/NA10HD Integration Hotspots
| Chr | Start | Stop | Length (bp) | VIS Count | Gene within 50 kb |
|---|---|---|---|---|---|
| chr1 | 181178372 | 181179843 | 1,471 | 5 | Cnih4//Nvl//Wdr26 |
| chr2 | 98662251 | 98667100 | 4,849 | 6 | |
| chr2 | 120513022 | 120514694 | 1,672 | 4 | 4931402G19Rik//Capn3//Zfp106 |
| chr4 | 129171134 | 129173005 | 1,871 | 8 | C77080//Fndc5//Hpca//S100pbp//Yars |
| chr4 | 134106904 | 134167961 | 61,057 | 6 | Aim1l//Catsper4//Cd52//Cep85//Gm7534//Sh3bgrl3//Ubxn11 |
| chr5 | 92157615 | 92160502 | 2,887 | 4 | U90926//Uso1 |
| chr6 | 84878244 | 84879506 | 1,262 | 4 | Exoc6b |
| chr7 | 66805807 | 66806746 | 939 | 4 | Adamts17//Cers3 |
| chr8 | 91217374 | 91218692 | 1,318 | 7 | Rpgrip1l |
| chr9 | 3000042 | 3035037 | 34,995 | 10 | Mir101c |
| chr11 | 96792129 | 96793084 | 955 | 4 | Cbx1//Gm11517//Nfe2l1//Skap1//Snx11 |
| chr13 | 55036100 | 55037292 | 1,078 | 6 | Hk3//Uimc1//Unc5a |
| chr14 | 19416508 | 19419195 | 2,687 | 5 | |
| chr15 | 89362301 | 89366163 | 3,862 | 6 | Adm2//Klhdc7b//Lmf2//Miox//Ncaph2//Odf3b//Sbf1//Sco2//Syce3//Tymp |
| chr17 | 64308442 | 64310543 | 1,985 | 7 | Pja2 |
A hotspot was defined as at least four integration sites within a 100 kb window at a certain genomic location. The size and number of integration site were shown for a total of 15 hotspots. The genes that were within 50 kb of the hotspots are listed, which include no oncogene or tumor suppressor genes.