Table 1.
Echocardiographic Analysis of Diastolic Function: Transmitral Doppler Parameters at 3 Weeks after TAC in Mice Pretreated with Lenti-shUBC9 or Lenti-shRNF4
| Index | Sham (n = 10) | TAC (n = 10) | +Lenti-shUBC9 (n = 10) | +Lenti-shRNF4 (n = 10) | +Lenti-scramble (n = 11) |
|---|---|---|---|---|---|
| LV mass (mg) | 1.28 ± 0.15 | 2.06 ± 0.25* | 1.43 ± 0.11# | 2.24 ± 0.25# | 1.92 ± 0.22* |
| E (cm/s) | 70.83 ± 10.79 | 74.50 ± 9.93 | 70.78 ± 9.51 | 84.84 ± 9.11 | 76.45 ± 8.38 |
| A (cm/s) | 49.66 ± 9.15 | 41.10 ± 9.33 | 49.79 ± 9.46 | 43.51 ± 11.47 | 46.34 ± 9.52 |
| E/A | 1.42 ± 0.15 | 1.87 ± 0.14* | 1.46 ± 0.13# | 2.05 ± 0.27# | 1.81 ± 0.19* |
| IVCT (ms) | 11.14 ± 1.64 | 12.60 ± 1.67 | 10.65 ± 1.36# | 15.58 ± 0.83# | 12.65 ± 2.10 |
| IVRT (ms) | 16.12 ± 1.38 | 17.45 ± 1.73 | 16.17 ± 1.53 | 20.01 ± 2.85# | 17.67 ± 1.16 |
| DT (ms) | 49.93 ± 5.11 | 46.29 ± 4.37 | 44.73 ± 4.04 | 47.35 ± 4.46 | 45.37 ± 6.27 |
| Tei index | 0.55 ± 0.01 | 0.65 ± 0.02* | 0.60 ± 0.01# | 0.75 ± 0.04# | 0.67 ± 0.02* |
All data are expressed as mean ± SD. n represents the number of mice used in each group. *p < 0.05 versus sham; #p < 0.05 versus +lenti-scramble. TAC, transverse aortic constriction; LV, left ventricular; E, the peak velocities of early rapid filling; A, the peak velocities of late filling; E/A, the ratio of peak velocity of early to late filling of mitral inflow; IVCT, isovolumetric contraction time; IVRT, isovolumic relaxation time; DT, deceleration time of early filling of mitral inflow; Tei index, calculation by the formula (IVRT + IVCT)/ET; +lenti-shUBC9, TAC+lentiviral vector carrying shRNA UBC9; +lenti-shRNF4, TAC+lentiviral vector carrying shRNA RNF4; +lenti-scramble, TAC+lentiviral vector carrying scramble RNA.