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. 2017 Jan 28;25(3):634–653. doi: 10.1016/j.ymthe.2016.12.015

Figure 1.

Figure 1

Progressive Restriction of Chrnb4-EGFP Expression in Developing Photoreceptors throughout Retinogenesis

(A–FII) Transversal sections of retinas at different developmental stages were collected from Chrnb4-EGFP reporter mice. (A–BII) EGFP detection is shown in round and elongated cells adjacent to the basal and to the most apical retinal region in E12 and E14 retinas. (B) White arrows indicate spindled cells in the central part of the retina expressing weak EGFP (probably progenitors). (C–CII) The EGFP expression is more restricted at the edge of the retinal apical side of E15.5 retinas. Few round cells at the basal retinal side still show EGFP expression (white arrows). (D–EII) In E18 and PN0 retinas, EGFP expression is exclusively restricted to the developing outer nuclear layer (dONL) in which photoreceptors differentiate. (F–FII) EGFP is expressed in a subset of photoreceptor cells at PN6, not in all photoreceptors. (F) The white arrow indicates the margin of the dONL. DAPI, nuclear staining (blue); Ba, basal; Ap, apical; PN, post-natal day; E, embryonic day. Scale bars, 20 μm.