Table 1. Primer details and genetic diversity estimates per locus as calculated from the captive Poicephalus robustus data set used in the current study.
The standard error for the average number of alleles is provided in parentheses. The values in superscript indicates the locus’ rank for the specific measure (1 = excellent and 16 = poor). It should be noted that Prob15 is reportedly Z-linked (Pillay et al., 2010) which could influence the null allele frequency.
| Locus | Primer sequence (5′–3′) | Average number of alleles (NA) | Allelic richness (Ar) | Probability of identity (PID) | Probability of exclusion (one parent known; PE2) | Polymorphic information content (PIC) | Null allele frequency as % (Na): | Inbreeding coefficient (FIS) | Hardy-Weinberg deviation (p-value) | Locus rank |
|---|---|---|---|---|---|---|---|---|---|---|
| Prob17 | F: TGAACATGACTTATTTGTCTAGTCATACCTAATCC | 17 (3.559) | 221 | 0.0181 | 0.6581 | 0.8881 | 0.0223 | 0.017 | 0.045 | 1 |
| R: TTCCAAGGAGTAATATACAGATAATTGCTTCTACA | ||||||||||
| Prob31 | F: GCTGCAGTACAGGCAGTCTTTG | 5.25 (1.109) | 6.9975 | 0.082 | 0.4042 | 0.7463 | 0.001 | −0.058 | 0.096 | 2 |
| R: CCCATGGCAGAAATTACAGTGA | ||||||||||
| Prob26 | F: GATCCCCAAAACAGATGAGTCT | 7.25 (1.436) | 9.8773 | 0.0884 | 0.3704 | 0.7234 | 0.001 | −0.109 | 0.188 | 3 |
| R: GTTTCTTGATTCAGATTGGAGGCTGATG | ||||||||||
| Prob30 | F: ACACTGAACCATGTCACACAAG | 6 (0.707) | 5.9978 | 0.0813 | 0.3973 | 0.7512 | 0.0374 | 0.044 | 0.0001* | 4 |
| R: GATCAGAAGGCTGCTTTGC | ||||||||||
| Prob23 | F: CACCAGTCATGACAGATAAT | 5 (1.08) | 5.9977 | 0.1065 | 0.3415 | 0.7075 | 0.012 | −0.091 | 0.034 | 5 |
| R: AGTATAAATTCAGCCTAGTTATGT | ||||||||||
| Prob25 | F: GATCCAGTGTGAAGCTAAAACAAGG | 4.75 (0.629) | 5.9469 | 0.1136 | 0.3306 | 0.6917 | 0.001 | −0.028 | 0.695 | 6 |
| R: GTTTCTTAAGGTAGATGTGGAGTGTAG | ||||||||||
| Prob18 | F: GATCATTGAGAACTATTTGGAAG | 4.25 (0.479) | 510 | 0.1127 | 0.3277 | 0.6946 | 0.001 | 0.035 | 0.198 | 7 |
| R: GTTTCTTATCAGTTGAACGCGAGAA | ||||||||||
| Prob06 | F: TCCAACCCACCTGAATTATCCAT | 6 (1.414) | 7.9574 | 0.1979 | 0.2139 | 0.5669 | 0.001 | −0.022 | 0.606 | 8 |
| R: GTTTCTTAGCTCCAATTCCGGGCTCT | ||||||||||
| Prob09 | F: GAACGTTTGTAGGGATAGTCCAC | 7.25 (1.493) | 10.8332 | 0.19910 | 0.19810 | 0.5610 | 0.066 | 0.146 | 0.003* | 9 |
| R: GTTTCTTACCGTGTCCACCCCTTATTCG | ||||||||||
| Prob15 | F: GTGTCCCAGCCAGACCCAAT | 5.5 (1.323) | 66 | 0.1358 | 0.3038 | 0.6568 | 0.1869 | 0.439 | 0* | 10 |
| R: TCAGGTGTCCTGTCTCTGCTTCC | ||||||||||
| Prob01 | F: TGCTCCCCATTCTACAGGTC | 3 (0.408) | 3.99914 | 0.20711 | 0.18611 | 0.55911 | 0.0585 | 0.129 | 0.016 | 11 |
| R: TGTTTCCATAATTTGGCTTGC | ||||||||||
| Prob29 | F: CAACACTGTGTATGCCCATGC | 3.75 (0.629) | 413 | 0.33813 | 0.10813 | 0.41513 | 0.001 | −0.1 | 0.134 | 12 |
| R: GTTTCTTGTTTGGACCCAGCAATCACC | ||||||||||
| Prob34 | F: GGTGCTGGAAGGTGGCTTCT | 4 (0.408) | 4.99911 | 0.36314 | 0.09514 | 0.39214 | 0.001 | −0.055 | 0.004 | 13 |
| R: GCTTTGGCTGGTGGTCCATT | ||||||||||
| Prob28 | F: GATCAAGGTATCATTAATAAGC | 3 (0.707) | 4.95712 | 0.2812 | 0.16712 | 0.47512 | 0.0817 | 0.14 | 0.001* | 14 |
| R: GAGCTCTCATTGTATGTCAA | ||||||||||
| Prob35 | F: ATTGCTGTATTGTGGGTAGG | 2.5 (0.5) | 3.99515 | 0.55715 | 0.03415 | 0.24615 | 0.001 | 0.055 | 0.067 | 15 |
| R: GATCAGCTCTTCACAGGAAT | ||||||||||
| Prob36 | F: GATCAAAAGCTATCTGACTGGACA | 1.75 (0.25) | 216 | 0.5916 | 0.03216 | 0.22116 | 0.1258 | 0.471 | 0.001* | 16 |
| R: GTTTCTTCCATATTCTCATTTGCTTTC | ||||||||||
| Mean | 6.813 (1.089) | 6.910 (1.149) | – | – | 0.581 (0.047) | 3.62 (1.37) | 0.047 | – |
Note:
*
p-value < 0.003.