Figure 2.

Differentiation of MNs with a Single SeV Vector Encoding Lhx3, Ngn2, and Isl1

(A) Outline shows the experimental procedure to generate MNs from HB9-EGFP knockin human iPSCs using a single vector, SeV-Lhx3-Ngn2-Isl1 (SeV-L-N-I). (B) Immunofluorescence staining of differentiated cells for HB9, Tuj1, MAP2, and ChAT is shown. Scale bars, 20 μm. (C) The percentages of HB9-positive and Tuj1-positive cells per total cells on day 14 were 5.3% ± 1.5% and 6.2% ± 1.6%, respectively. Error bars are SEM (n = 3). (D) The qPCR analysis of the differentiated cells on days 0 and 14 for HB9, ChAT, and MAP2 is shown. Student’s t test was used for statistical comparison (*p < 0.05). Error bars are SEM (n = 3).