Figure 4.
Sensitivity of Detection of Transduced Ec dhfr Reporter Cells
(A) HEK293 dhfr cells were injected subcutaneously in Matrigel (150 μL) at concentrations of 3 × 106, 3 × 105, and 3 × 104 cells 24 hr prior to radiotracer administration and PET imaging. The Matrigel was harvested and measured on a gamma counter. Error bars, SEM (n = 3). There was a statistically significant difference between muscle control and 3 × 106 and 3 × 105 cell grafts (p < 0.05 Student’s t, two-tailed). (B) Representative images in three planes of the shoulder area containing 3 × 105Ec dhfr cells.