Table 1.
qPCR Primers
| Gene | Forward and Reverse Primer Pair Sequences | Annealing Temperature (°C) |
|---|---|---|
| Mouse Collagen 1A1 | TTCACCTACAGCACGCTTGTG | 58 |
| GATGACTGTCTTGCCCCAAGTT | ||
| Mouse CTGF | CAAAGCAGCTGCAAATACCA | 58 |
| GGCCAAATGTGTCTTCCAGT | ||
| Mouse TIMP- 1 | GCAACTCGGACCTGGTCATAA | 58 |
| CGGCCCGTGATGAGAAACT | ||
| Mouse IL-6 | GAGGATACCACTCCCAACAGACC | 58 |
| AAGTGCATCATCGTTGTTCATACA | ||
| Mouse TGFβ 1 | CTCCCGTGGCTTCTAGTGC | 58 |
| GCCTTAGTTTGGACAGGATCTG | ||
| Mouse α SMA | TCAGCGCCTCCAGTTCCT | 58 |
| AAAAAAAACCACGAGTAACAAATCAA | ||
| Mouse Slpi | GTGGAAGGAGGCAAAAATGA | 58 |
| GACATTGGGAGGGTTAAGCA | ||
| Mouse HAMP2 | CTGCCTGTCTCCTGCTTCTC | 58 |
| GCAGATGGGGAAGTTGATGT | ||
| Mouse G6pC | TCTGTCCCGGATCTACCTTG | 58 |
| GTAGAATCCAAGCGCGAAAC | ||
| Mouse Thrsp | ACGGAGCCCCTGATCTCTAT | 58 |
| GGCTTCTAGGTCCAGCTCCT | ||
| Mouse GAPDH | GCACAGTCAAGGCCGAGAAT | 58 |
| GCCTTCTCCATGGTGGTGAA | ||
| Mouse angiopoietin 1 | AGGCTTGGTTTCTCGTCAGA | 56 |
| TCTGCACAGTCTCGAAATGG |