Figure 1.

Histological Analysis for Detection of GFP

(A) Nineteen weeks after intravitreal injection of AAV, eyes were enucleated, sectioned, and stained for GFP. GFP expression was compared among a control group, group VIT (two eyes receiving VIT 1 month before AAV injection), and group VIT+ILM (two eyes receiving VIT and ILM peeling 1 month before AAV injection) at the macula and temporal fovea (1,500–2,000 μm temporal from the macula). White arrows indicate the RPE, which showed autofluorescence. Little GFP expression was detected in the control group or group VIT. Strong GFP expression was detected in group VIT+ILM (original magnification ×200; scale bar, 200 μm). (B) GFP expression areas (in pixels) were determined using ImageJ software and Photoshop software. The area of GFP expression in group VIT+ILM was broader than in the control group or group VIT.