Figure 3.

6BIO Enhances SSO Function in the Cell Nucleus

(A) Photomicrographs of HeLa-EGFP-654 cells treated with DMSO control (Con), 1 μM non-targeting oligo (Con-ASO), 1 μM SSO (SSO), 2 μM 6BIO (6BIO), a combination of 1 μM control oligo and 2 μM 6BIO (Con-ASO/6BIO) or 1 μM SSO and 2 μM 6BIO (SSO/6BIO) as indicated, for 2 days prior to imaging. Scale bar, 50 μM. (B) Representative experiment showing the analysis of flow cytometric data obtained from HeLa-EGFP-654 cells treated as in (A). The EGFP subset gate was defined by defining the non-treated control cells as EGFP-negative. The profiles shown are typical of several independent experiments. (C) The graph represents the combined flow cytometric data analysis of three different experiments. The y axis indicates the percentage of cells in the EGFP subset window. Data are expressed as the mean ± SD. n = 3. ***p < 0.001 (Student’s t test).