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. 2017 Jan 4;25(1):44–53. doi: 10.1016/j.ymthe.2016.10.007

Figure 2.

Figure 2

Hematopoietic Differentiation from Genetically Modified RhiPSCs

(A) A summary graph of flow cytometry analysis comparing hematopoietic differentiation from RhiPSC-hΔCD19 versus unedited clones. There was no significant difference between groups (Student’s t tests, p = 0.6620, n = 3). Error bars indicate SEM. (B and C) CD34+CD45+ cells derived from RhiPSC-hΔCD19 (B) and RhiPSC-GFP (C) clones maintained transgene expression after differentiation. (D) A representative image of transgene (GFP) expression from a CFU-M derived from hematopoietic differentiation of the RhiPSC-GFP-21 clone. A total of 24 colonies were produced in this differentiation experiment and 24 of 24 were GFP positive.