Table 1.
Summary of CRISPR/Cas9-Mediated Gene Editing in RhiPSCs
| Parental iPSC Clone | Reporter Gene | Targeted Insertiona | Single-Copy Integrationb | Off-Target Mutationsc |
|---|---|---|---|---|
| ZG15-M11-10 | hΔCD19 | 8/8 | 2/8 | 1/2 |
| GFP | 14/14 | 5/9 | 1/4 | |
| ZG32-3-4 | hΔCD19 | 4/4 | 2/4 | 1/1 |
| GFP | 4/4 | 1/4 | 1/1 | |
| ZH26-HS41 | hΔCD19 | 5/5 | 2/5 | 1/2 |
| Total | 35/35 (100%) | 12/30 (40%) | 5/10 (50%) |
a
Clones with targeted integration/transgene-positive clones, based on PCR analysis.
b
Monoallelic clones without random integration/clones with targeted integration, based on Southern blot analysis. The rest of colonies had additional random integration event or events, as well as the targeted integration event.
c
Clones with off-target mutations/clones with single-copy integration, based on Sanger sequencing.