Table 1.
Study Design
| Rapa (mg/kg) | Borta (mg/kg) | Predb (mg/kg) | Number of Mice Assayedc |
|||
|---|---|---|---|---|---|---|
| Pre-IS | IS-4w | IS-8w | ||||
| Experiment 1 | ||||||
| non-IS | – | – | – | 9 | 9 | 9 |
| RH | 2.0 (i.p.) | – | – | 15 | 15 | 13 |
| BH | – | 2.0 (i.p.) | – | 5 | 5 | 5 |
| PH | – | – | 0.5 (i.p.) | 5 | 5 | 5 |
| BH + PL | – | 2.0 (i.p.) | 0.5 (i.p.) | 5 | 5 | 5 |
| BL + PL | – | 1.0 (i.p.) | 0.5 (i.p.) | 4 | 4 | 4 |
| BL + PH | – | 1.0 (i.p.) | 0.75 (i.p.) | 4 | 4 | 4 |
| RH + PL | 2.0 (i.p.) | – | 0.5 (i.p.) | 5 | 4 | 3 |
| RH + PH | 2.0 (i.p.) | – | 0.75 (i.p.) | 5 | 4 | 3 |
| Experiment 2 | ||||||
| RH + PL | 2.0 (i.p.) | – | 0.5 (i.p.) | 13 | 13 | 10 |
| RL + PL | 1.0 (i.p.) | – | 0.5 (i.p.) | 6 | 6 | 6 |
| RL + PL(O) | 2.0 (i.p.) | – | 0.5 (oral) | 5 | 5 | 5 |
| non-IS | – | – | – | 5 | 5 | 5 |
WT mice were immunized with an i.p. injection of 1 × 1010vg/kg rAAV9 vector and then treated with different IS agents starting 5 weeks pi. Pre-IS, prior to IS; IS-4w, 4 weeks on IS treatment; IS-8w, 8 weeks on IS treatment; non-IS, non-treated controls; R, Rap; B, Bort; P, Pred; H, high dose; L, low dose.
a
Every other day.
b
Daily.
c
Decreases in study subjects over time were due to unexpected loss from blood draw or injections.