Figure 4.

Intracellular Distribution of Fluorescent TPP1

Wild-type mice were treated with 1 mg Alexa-647-labeled rhTPP1 via IT administration. Then 18 hr later, animals were killed, and brains were analyzed by confocal microscopy to measure the distribution of fluorescently labeled TPP1. Images were captured using a 40× objective. Blue, DAPI; red, Alexa-647 rhTPP1. Note that images were chosen for each brain region to illustrate intracellular distribution and are not meant to be representative of each respective brain region as a whole.