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. 2016 Dec 24;4:50–61. doi: 10.1016/j.omtm.2016.11.004

Figure 2.

Figure 2

CD163-Positive Liver Macrophages in Rats on the Standard Diet versus the HFr Diet

(A and B) Anti-CD163 antibody staining of Kupffer cells/infiltrating macrophages using immunohistochemistry of fixed paraffin-embedded liver sections from rats fed the (A) STD or (B) HFr diet for 2 weeks. White scale bars represent 400 μm. (C and D) Fluorescence microscopic analysis of formalin-fixed cryosections of rat liver 1 hr after i.v. injection of (C) the anti-CD163-dexamethasone conjugate and (D) the control IgG-dexamethasone conjugate in rats on the STD. Anti-mouse IgG-Alexa Fluor 488 (green) was used for detection of the conjugates. Staining of the CD163-dexamethasone conjugate is in accordance with uptake in Kupffer cells (arrowheads). Scale bars represent 20 μm.