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. 2016 Dec 24;4:27–38. doi: 10.1016/j.omtm.2016.12.002

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© 2016 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Stimulation of BDC2.5 CD4⁺ T Cells by Transduced DAPg7 Cells

(A–C) Comparison of constructs with mixed epitopes (NEO and NEM) and segregated epitopes (NMS) for CD25 upregulation (A) and T cell division (B), with representative dot plots (C), gated on live CD4⁺ singlets. (D and E) Comparison of mixed epitope constructs (NEO and NEM) without or with four types of TSs for CD25 upregulation (D) and T cell division (E). Data show the mean ± SD from three biological replicates (three donor transgenic mice) and from one of two similar experiments. Paired t test analysis: *p < 0.05, **p < 0.01, ***p < 0.005.