Table 1.
DNA Amounts in AAV Pseudo-vectors and Full AAV Particles
| Vectors | Density (g/cm3) | Genome Size (nt) | DNA Content (ng/1E+13 Particles) |
Percenta | |
|---|---|---|---|---|---|
| Theoretical Value | Experiment Value | ||||
| nAAV8 | 1.32–1.34 | – | – | – | – |
| aAAV8 | 1.32–1.34 | < 1,000 | – | 193 ± 46 | 0.96 |
| sAAV8 | 1.32–1.34 | < 1,000 | – | 142 ± 51 | 0.51 |
| AAV8-TTR-hF8 | 1.40–1.47 | 5,080 | 27,847 | 17,543 ± 2,240 | 63.0 |
| AAV8-hHC | 1.38–1.44 | 3,672 | 20,129 | 17,588 ± 3,291 | 87.4 |
a
Percent is the ratio of DNA amount in sAAV8 or nAAV8 to their theoretical value with 100% of AAV vectors containing the intact genome.