Figure 7.

Metabolic incorporation of BCNSia into zebrafish embryos and subsequent labelling with fluorogenic Oregon green tetrazine dye. A) Schematic representation of expected metabolic pathway for the incorporation of BCNSia into cell‐surface glycans and subsequent labelling. CMAS: cytidine monophosphate N‐acetylneuraminic acid. CMP: cytidine monophosphate. B) Schematic representation of labelling protocol. Zebrafish embryos were injected with BCNSia and then with fluorogenic tetrazine in the caudal vein. C) Projection images of 48 hpf (hours post‐fertilisation) embryos treated with BCNSia or vehicle; fluorogenic tetrazine was coinjected with AF647‐NH₂ to map the vasculature. Scale bar: 200 μm. Reproduced in part, with permission, from ref. 77. Copyright: 2015, John Wiley & Sons, Inc.