Figure 1.

TRPV4 mRNA and protein expression in human gastric tissues and AGS cells. A, TRPV4 and GAPDH mRNA were examined with (+) and without (‐) RT reaction. The expected sizes of the amplified fragments for human TRPV4 and GAPDH were 543 and 983 bp, respectively. TRPV4 mRNA was detected in human gastric tissues but not in AGS cells. B, TRPV4‐ir was observed in human gastric epithelium. The reaction was diminished without anti‐TRPV4 antibody (Ab) or an antigenic peptide (Ag) (absorption experiment). Bars indicate 50 μm