Figure 4.

TRPV4 DNA methylation silencing in the human normal gastric epithelial cell line GES‐1 with Helicobacter pylori coculture. A, TRPV4 mRNA expression levels in GES‐1 with coculture of two different types of H. pylori (NCTC and 193C). Relative expression levels significantly decreased in a time‐dependent manner with coculture of both H. pylori types (*P<.01 vs control). B, Western blotting (WB) indicated that TRPV4 protein expression also decreased with coculture of H. pylori in a time‐dependent manner. C, MSP indicated that methylated PCR products (M) were observed in coculture GES‐1 samples, but not in the control