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. 2017 Jan 13;214(2):655–667. doi: 10.1111/nph.14414

Table 3.

Estimates of in vivo Rubisco oxygenase and carboxylase activities made from measurements of gas exchange and chlorophyll fluorescence under ambient (21%) or low (2%) O2

Wild‐type 1a3b 1a2b 1AAt 1AAtMOD S2Cr
GA Low (μmol m−2 s−1) 9.48 ± 0.56a 4.58 ± 0.4c 5.69 ± 0.53c 8.73 ± 0.09ab 8.57 ± 0.64ab 6.72 ± 0.49bc
GA amb (μmol m−2 s−1) 6.17 ± 0.36a 2.98 ± 0.25d 3.67 ± 0.36 cd 5.6 ± 0.15ab 5.49 ± 0.42ab 4.36 ± 0.38bc
J NADPHlow (μmol m−2 s−1) 18.9 ± 1.1a 9.1 ± 0.8c 11.4 ± 1.1c 17.5 ± 0.2ab 17.1 ± 1.3ab 13.4 ± 0.9bc
J NADPHamb (μmol m−2 s−1) 19.9 ± 0.9a 9.2 ± 0.7c 11.9 ± 0.9bc 18.4 ± 0.2a 18.2 ± 1.4a 14.1 ± 1b
V o (μmol m−2 s−1) 2.21 ± 0.13a 1.06 ± 0.11b 1.34 ± 0.12b 2.09 ± 0.06a 2.06 ± 0.17a 1.57 ± 0.07ab
V c (μmol m−2 s−1) 7.27 ± 0.43a 3.52 ± 0.29d 4.35 ± 0.41c 6.64 ± 0.12ab 6.52 ± 0.49ab 5.15 ± 0.42bc
V o/V c 0.304 ± 0.002a 0.302 ± 0.013a 0.307 ± 0.008a 0.313 ± 0.015a 0.316 ± 0.012a 0.307 ± 0.011a

Arabidopsis thaliana plants (35–40 d old) were measured under 300 μmol photons m−2 s−1, and ambient CO2 of 300 μmol mol−1 as in Bellasio et al. (2014). For wild‐type, 1a3b and 1a2b values are the means ± SE of measurements made on individual leaves from five different rosettes. For transgenic lines, values are means ± SE of measurements made on 15 rosettes, five from each of the three lines. Values are followed by letters indicating significant difference (< 0.05), as determined by ANOVA followed by Tukey's HSD tests. Values followed by the same letter are not significantly different. GA low, gross photosynthetic rate (A+R d) under 2% O2 (2%); GA amb, gross photosynthetic rate under 21% O2; J NADPHlow, NADPH produced for photosynthesis (derived from electron transport rate) under 2% O2; J NADPHamb, NADPH produced for photosynthesis under 21% O2; V o, Rubisco oxygenation rate; V c, Rubisco carboxylation rate.