Figure 2.

Expression and production of properdin and fH by human DCs and tolDCs. Dendritic cells were harvested after 6 days of culture, washed and stimulated with IFN‐γ or LPS, after which mRNA was isolated followed by cDNA synthesis. The transcript levels of (A) Properdin (CFP) (B) Factor H (CFH) were determined by RT‐PCR. GAPDH mRNA expression from the same samples was used as an endogenous reference gene (relative mRNA expression). Data are shown is mean + SD of 8 independent experiments performed with eight different donor samples.* one‐tailed t‐test p≤0.047,**p≤0.082 (C) DCs were cultured on 8‐well chamber slides and either untreated or stimulated with IFN‐γ followed by incubation with Brefeldin. The cells were then fixed and permeabilized, followed by incubation with anti‐fP or anti‐fH followed by detection with GaM‐Alexa⁴⁸⁸. Fluorecent images are from a single experiment representative of experiments performed with three different donor samples. Scale bar 25 μm.