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. 2017 Feb 1;102(2):228–244. doi: 10.1113/EP085910

Figure 4. In vitro release of ATP with heating and cooling.

Figure 4

Erythrocyte, plasma and serum samples were incubated in water‐baths set at 39, 33 (control), 27 and 20°C. Release of ATP was measured in blood samples taken from 12 subjects. *Significantly different from 33°C (control). Data are shown as means ± SD. Separately, confluent cultures of human umbilical vein endothelial cells (HUVECs) were incubated in water‐baths set at 37 (control), 39 and 23°C (room temperature) for 30 min. Release of ATP was measured in duplicate from three independent primary HUVEC cultures from different individuals. Data are shown as means ± SD.