Figure 1.

Target Sites of gRNAs in the Promoter of the Mouse Lama1 Gene

The figure illustrates part of the sequence of the Lama1 promoter. The start signal (ATG) is highlighted in yellow. The positions of two pairs of primers are highlighted in gray and in brown. The first pair produces a 1,261-bp amplicon and the second pair a 1,602-bp amplicon. The positions of six different gRNA target sequences are highlighted in various colors. The PAM sequence is underlined. The positions of two transcription factors (KLF4 and SP1) are also highlighted, respectively, in green and in light blue. These two sites are partially overlapped, respectively, the gRNA4 and gRNA5 target sites.