Figure 3.

Increased Expression of Lama1 by dCas9-VP160 and Individual gRNA1–5

C2C12 cells were transfected with five different pAC154 each coding for dCas9-VP160 and for a gRNA (i.e., gRNAs 1–5). The control cells (C) were transfected with a plasmid coding for eGFP. The Lama1 mRNA was quantified by qRT-PCR and expressed either as the number of copies per microgram of total RNA (left axis and white bars) or normalized with HPRT mRNA (A, right axis, black bars) or GAPDH mRNA (B, right axis, black bar).