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. 2016 Dec 10;6:68–79. doi: 10.1016/j.omtn.2016.11.004

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© 2016 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Combinations of gRNA3 and gRNA4 with dCas9-P300 Further Increased the Expression of the Lama1 mRNA

The C2C12 cells were transfected with TransfeX with two plasmids coding for dCas9-P300 and for gRNA3 and gRNA4. The control cells (C) were transfected with a pAC154 plasmid containing the dCas9VP160 but not coding for a gRNA. The Lama1 mRNA was quantified by qRT-PCR and expressed either as the number of copies per microgram of total RNA (left axis and white bars) or normalized with HPRT mRNA (A, right axis, black bars) or GAPDH mRNA (B, right axis, black bar). The figure illustrates three independent replications of the experiment.