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. 2016 Dec 10;6:221–232. doi: 10.1016/j.omtn.2016.11.007

Figure 1.

Figure 1

Schematic Diagram of Plasmid DNA of RRVs Expressing shRNA, miRshRNA, and yCD2-miRshRNA

shRNA, miRshRNA, and yCD2-miRshRNA in various configurations inserted downstream of the 3′ UTR at Mlu I and Not I restriction sites. The filled arrowheads indicate primer set (5–127 and 3–37) used for endpoint PCR to evaluate the integrity of insert in proviral DNA. The open arrowheads indicate primer set used for qRT-PCR to measure the viral genome expression in the supernatant of RRV infected cells. The numbers indicate the lengths in base pair of inserts or PCR products for each configuration.