Figure 6.

Downregulation of PDL1 by RRV-miRPDL1 Restores PHA-Stimulated T Cell Activation In Vitro

(A) PHA-induced PBMCs were cultured alone or co-cultured with Mel 103 cells or Mel 103 cells overexpression PDL1 (Mel 103/LV-PDL1). IFNγ production in the supernatant was measured by ELISA. (B) PHA-induced PBMCs were cultured alone or co-cultured with LN-18 parent cells or LN18 cells infected with RRV-miRPDL1 (LN18/KD). IFNγ production in the supernatant was measured by ELISA. (C) PHA-stimulated PBMCs were cultured alone or co-cultured with RRV-miRGFP infected MDA-MB-231BR cells in the presence or absence of anti-PDL1 and anti-PD1 antibody and compared to that co-cultured with RRV-miRPDL1 infected MDA-MB-231BR cells. The intracellular IFNγ expression in the CD3⁺/CD8⁺ gated population was analyzed by flow cytometry. The values present percentage of IFNγ⁺ cells from one of multiple experiments.