Figure 1.

The infiltration of Tregs into the CNS after ICH. (a) Representative FACS plots showing that CD45^hiCD3⁺CD4⁺ cells were defined as brain-invading T helper cells and further analyzed to identify Tregs. FSC-A indicates the forward scatter channel area, and SSC-A indicates side scatter channel area. (b) Temporal changes in brain-invading CD4⁺T cells and Tregs after ICH, and Sham-operated mice that were analyzed on day 4 post-hemorrhage. (c) Representative FACS plots showing that CD4⁺Foxp3⁺ cells are considered Tregs in spleen. (d) The proportions of Foxp3⁺ cells in CD4⁺ cells in the brain and the spleen were separately analyzed at 1, 4, 7 and 14 d after ICH (4 individual experiments with four pooled animals per group in each experiment; *p < 0.05 versus the sham group in brain, ^#p < 0.05 versus the sham group in spleen).