Figure 1.

Experimental procedures and animal groups. Mice submitted to transient middle cerebral artery (MCA) occlusion (MCAO) that were treated with vehicle or memantine (4 or 20 mg/kg/d) starting at 72 h after reperfusion over up to 28 d were used for (a) behavioral tests (Rotarod, tight rope, and Barnes maze tests) and tract tracing studies, (b) histochemistry and conventional immunohistochemistry, and (c) ELISA and Western blots. Numbers of animals evaluated for each group and time-point of animal sacrifice (in days post-treatment onset, dpt) are also shown. BL, baseline.