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Maximum intensity projection of a two-photon fluorescence microscopy z-stack from the surface of the motor cortex (a, scale 50 µm) and side angle (b, scale 100 µm) with red showing vascular endothelial cells expressing tdTomato and green showing intravascular Oregon green. (c) and (d) (scale 10 µm) shows tdTomato labeling in the absence of Oregon green labeling (blue arrows), suggesting a new vessel.
