Figure 1.

Verification of Lamin and Vimentin as LC-18-Associated Protein Targets

(I) Co-staining of the tissue sections with Alexa Fluor 405-labeled anti-lamin (A) and anti-vimentin (B) antibodies and Cy-5-labeled aptamer LC-18. (1) H&E staining of the adjacent section, (2) staining with correspondent Alexa Fluor 405-labeled antibody, (3) Cy-5-labeled aptamer LC-18, (4) overlay, and (5) histogram of fluorescence intensity are shown. Magnification, 40×. (II) Flow cytometry histograms indicating binding of aptamer LC-18 to lung adenocarcinoma cells and its replacement with anti-lamin (A) and anti-vimentin (B) antibodies. Red curve corresponds to intact lung adenocarcinoma cells, blue curve corresponds to cells bound with the aptamer LC-18 (50 nM), and green curve corresponds to the same sample after incubation with 2 ng μL⁻¹ antibodies for 30 min with shaking at 37°C.